Can we make an ISO of two drives (D and E)

[doer]

I'd like to throw my actual Win7 Live USB content to Ventoy. But I would first have to create an ISO file of my Win7 Live USB. Can we make an ISO image of two drives (D and E) as we have in the Live USB structure? This is my question.

I know I could throw to Ventoy "plain" Win7 ISO. Plain - I mean, without my settings, personalization, and my content. It's just a fresh ISO. But then, installing third-party programs is time-consuming and a pain in the neck to make settings to my liking.